Urine sample test strip

Urine analysis, also known as urinalysis, encompasses all the analytical tests carried out on a urine sample. It usually involves three forms of analysis:

  • Physical – Assessment of the color, cloudiness and concentration of the urine
  • Chemical – Examination of the chemical constituents of the urine using a test strip
  • Microscopic – this allows one to look for bacteria, cells and cell fragments
Check-up. Medical report and urine test strips - Image Copyright: Alexander Raths / Shutterstock
Check-up. Medical report and urine test strips – Image Copyright: Alexander Raths / Shutterstock


Urine analysis may be required as part of:

  • Overall health assessment: for routine medical examination, pre-surgery preparation, or upon hospital admission to screen for disorders including kidney and liver disease.
  • Medical Diagnosis: for those experiencing symptoms including abdominal pain, back pain, frequent or painful urination and blood in your urine.
  • Medical Monitoring: following the diagnosis of kidney disease to track both condition and treatment.
  • Specific testing. This includes pregnancy tests and drug screenings e.g. pregnancy tests measure the hormone human chorionic gonadotropin (HCG).

Urine analysis encompasses the following testing procedures:

Rapid Urine Test

This test involves dipping a test strip embedded with colored fields into a urine sample for a few seconds. Depending on the concentration of the particular substance being testing for, the fields on the test strip change color. By using the color table on the test package as reference, one can see if any compounds exhibit abnormal urinary levels.

The following can be analysed:

  • Colour: Fluid balance, diet, medicines, and diseases can affect this (e.g. Vitamin B, consumption of blackberries and beets).
  • Clarity: Substances including bacteria, blood, or sperm can make normal, clear urine cloudy.
  • Odor: Some diseases may cause a change in the odour of urine( e.g. diabetes can cause a sweet, fruity odour.)
  • Specific gravity: Indicates production of urine by checking the concentration of substances in the urine against water content (e.g. when fluid intake is high, the kidneys produce urine with a high level of water, which has a low specific gravity.)
  • pH:  normally ranges from approximately 5 to 7 but certain treatments affect this and treatment may be needed to prevent the formation of some types of kidney stones.
  • Protein: not normally present in the urine, but strenuous exercise, pregnancy, fever and some diseases (e.g. kidney disease and nephritis) may cause its appearance.
  • Glucose:  normally there is very little or no glucose in urine. When the kidneys are damaged and/or blood sugar level is very high (uncontrolled diabetes), urinary level increases.
  • Nitrites: bacteria that cause a urinary tract infection (UTI) possess an enzyme that converts nitrates to nitrites. Thus, urinary nitrites indicate a UTI.
  • Ketones: Fat is metabolised into ketones, which are expelled via urine. Large amounts of ketones in the urine may indicate diabetic ketoacidosis, a diet low in sugars and carbohydrates or starvation.

Urine Culture

This can be used to detect bacteria in the urine. A sample of midstream urine is incubated for 1-2 days with an appropriate medium to allow for bacterial growth. If any bacteria or fungi are present, there will be colony growth that will be studied based on size, form and color. Often urine cultures are used to test for a UTI then appropriate antibiotics are prescribed for its management.

Reviewed by Yolanda Smith, BPharm